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May 31, 2026Angewandte ChemieOpen Access

A Non‐Covalent 4Fe–4S/2Fe Interface in HydF Guides FeFe‐Hydrogenase Maturation

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Authors

GCGiorgio CasertaPCPrincess R. CabotajeAWArmel T. Waffo

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Overview

Randomized trial demonstrates the function of the HydF maturase in hydrogenase assembly, suggesting crucial interactions.

Key Points

  • This research aims to clarify the role of the HydF protein during the maturation process of [FeFe]-hydrogenase.
  • Used 57Fe nuclear resonance vibrational spectroscopy and selective isotopic labeling.
  • Conducted protein structure predictions to support findings about the [4Fe–4S] and [2Fe] arrangement.
  • Explored earlier biosynthetic steps related to the HydF maturase.
  • The [2Fe] subsite binds near the [4Fe–4S] cluster without a covalent cyanide bridge.
  • Spectroscopic and structural studies reconcile previous conflicting data about HydF's function.
  • The [4Fe–4S] cluster aids in forming the bridge for the [2Fe] site, assisting in biosynthetic assembly.

Cite This Study

Caserta et al. (2026) studied this question.

synapsesocial.com/papers/6a1bd2375783ba022b6fd93chttps://doi.org/10.1002/ange.8078898
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