Randomized trial shows improved detection of Aspergillus fumigatus in cystic fibrosis, suggesting optimization of culture methods.
Key Points
This analysis aimed to determine the best fungal culture media and incubation conditions for detecting Aspergillus fumigatus in cystic fibrosis sputum.
Analyzed 281 sputum samples from 74 adults with cystic fibrosis across two laboratories.
Compared growth rates of Aspergillus fumigatus on various culture media and incubation temperatures.
Evaluated the impact of oxygen levels on fungal growth.
Inhibitory mold agar significantly reduced Aspergillus fumigatus detection (aOR 0.78, 95% CI 0.68–0.89, P < 0.01).
30°C incubation compared to 37°C decreased positivity by 21% O2 (aOR 0.71, 95% CI 0.58–0.88, P < 0.01).
High oxygen levels (37°C 21% O2 5% CO2) decreased detection compared to lower oxygen conditions (aOR 1.82, 95% CI 1.41–2.36, P < 0.01).