Experimental work develops a new enzyme panel, exploring activity and stereochemistry in transformations.
In this study, we report the expression and characterization of a new panel of amine dehydrogenases (AmDHs) and amino acid dehydrogenases (AADHs). The panel of enzymes was initially screened against a library of candidate aldehyde and ketone substrates, in order to identify potential hits, using an NAD(P)H depletion assay. In some cases beneficial mutations from the literature were introduced to relevant sequences to improve activity. Finally some specific ketones (e.g., 17a , 18a , and 19a ) were studied in detail in order to characterize the stereochemistry of the transformations.
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Allen et al. (2026) studied this question.
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