What does this research mean for the field?

Hepatic fibrinogen/angiopoietin-related protein (HFARP) is a novel, liver-secreted protein present in circulating blood that protects vascular endothelial cells from apoptosis via an endocrine action independent of Tie1 or Tie2 receptors. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.ESTABLISHES_NEW_DIRECTION.

What question did this study set out to answer?

To identify, clone, and characterize the structure, tissue expression pattern, and vascular function of a novel angiopoietin-related protein.

March 7, 2000Biochemical JournalOpen Access

Hepatic expression, synthesis and secretion of a novel fibrinogen/angiopoietin-related protein that prevents endothelial-cell apoptosis

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To identify, clone, and characterize the structure, tissue expression pattern, and vascular function of a novel angiopoietin-related protein.
Isolated human (406-amino acid) and mouse (410-amino acid) cDNA from embryonic libraries using degenerate PCR.
Expressed recombinant protein in COS-7 cells to characterize secretion, glycosylation, and binding affinity to Tie1 and Tie2 receptors.
Assayed tissue-specific mRNA and protein distribution and measured anti-apoptotic effects on vascular endothelial cells.
HFARP mRNA and protein were specifically expressed in hepatocytes and found secreted into circulating blood.
Recombinant HFARP promoted vascular endothelial cell survival by inhibiting apoptosis without binding to Tie1 or Tie2 endothelial tyrosine kinase receptors.

Structured PICO

Population

Human and mouse embryonic cDNAs, human and mouse tissues, COS-7 cells, and vascular endothelial cells

Intervention

Recombinant hepatic fibrinogen/angiopoietin-related protein (HFARP)

Outcome

Effect on endothelial-cell apoptosis and binding to Tie1 or Tie2 receptors

Identification of HFARP, a novel liver-secreted protein that protects vascular endothelial cells from apoptosis, potentially acting via an endocrine mechanism.

Discussion

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Implication

HFARP may promote endothelial survival independent of Tie receptors in animals; leaves open its role as a novel endocrine factor for vascular research.

Cite This Study

Kim et al. (2000) studied this question.

synapsesocial.com/papers/6a1c05bc5b8f4ede65a96845 https://doi.org/10.1042/bj3460603

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Implication

HFARP may promote endothelial survival independent of Tie receptors in animals; leaves open its role as a novel endocrine factor for vascular research.

Hepatic expression, synthesis and secretion of a novel fibrinogen/angiopoietin-related protein that prevents endothelial-cell apoptosis

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Hepatic expression, synthesis and secretion of a novel fibrinogen/angiopoietin-related protein that prevents endothelial-cell apoptosis

Key Points

Structured PICO

Discussion

Implication

Cite This Study

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