A general survey of the reaction of neutral red upon a selected series of living animal organisms is made, and the rationale indicates that neutral red is an indicator of proteolytic enzyme action, whether this be intra‐ or extracellular. Cellular hydrolytic and cellular synthetic systems are thus manifested. The neutral‐red reaction also reveals the course of assimilation and secretion in organisms, the nature of fertilization, and the beginning of embryological differentiation.
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Vera Koehring (1930) studied this question.
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