Point Mutation Leu1014Phe (voltage-gated sodium channel/VGSC) gene in Aedes aegypti (L.) as Insecticide Resistance Marker Synthetic Pyrethroid in Palembang, Indonesia

Aedes aegypti is a vector of several pathogens including dengue fever/dengue hemorrhagic fever virus.Five hundred thousand dengue hemorrhagic fever new cases occur every year throughout the world.Vector control is an effective way to break the transmission; unfortunately constant insecticide used caused resistance.The purpose of research was to identify the Leu1014Phe point mutation in the VGSC gene of Ae.aegypti in Palembang.Population were all 3rd and 4th instar larvae of Ae.aegypti derived from breeding eggs obtained from villages of Bukit Kecil, Ilir Timur I and Sukarami sub district.Results showed that there has been Leu1014Phe point mutation.It can be concluded that synthetic pyrethroid insecticides resistance in Palembang on its Ae.aegypti was due to the target site mechanism.