Video microscopy provides a promising, fast, and reliable method for analyzing the mechanical beating behavior of iPSC-derived cardiomyocytes for basic research and cardiac safety toxicology.
Video microscopy is highlighted as a promising and efficient method for analyzing the mechanical beating behavior of iPSC-derived cardiomyocytes in disease modeling and toxicology screening.
A rapidly increasing number of papers describing novel iPSC models for cardiac diseases are being published. To be able to understand the disease mechanisms in more detail, we should also take the full advantage of the various methods for analyzing these cell models. The traditionally and commonly used electrophysiological analysis methods have been recently accompanied by novel approaches for analyzing the mechanical beatingbehavior of the cardiomyocytes. In this review, we provide first a concise overview on the methodology for cardiomyocyte functional analysis and then concentrate on the video microscopy, which provides a promise for a new faster yet reliable method for cardiomyocyte functional analysis. We also show how analysis conditions may affect the results. Development of the methodology not only serves the basic research on the disease models, but could also provide the much needed efficient early phase screening method for cardiac safety toxicology. This article is part of a Special Issue entitled: Cardiomyocyte Biology: Integration of Developmental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel.
Laurila et al. (Sat,) conducted a review in Cardiac diseases (iPSC models). Video microscopy for cardiomyocyte functional analysis vs. Traditional electrophysiological analysis methods was evaluated. Video microscopy provides a promising, fast, and reliable method for analyzing the mechanical beating behavior of iPSC-derived cardiomyocytes for basic research and cardiac safety toxicology.