Purpose: The present study aimed to define the epidermal moisturizing effects of human dermal stem/progenitor cell-derived conditioned medium (hDSPC-CM) on normal human epidermal keratinocytes (NHEKs). Methods: To investigate the epidermal moisturizing effects of hDSPC-CM on NHEKs, I measured 2,2-diphenyl-1picrylhydrazyl (DPPH) radical-scavenging activity and gene expression and performed cell viability assay and hyaluronic acid (HA)-enzyme-linked immunosorbent assay (ELISA). Results: In the present study, I elucidated the effects of hDSPC-CM on the mRNA expression of hyaluronan synthase 2 (HAS2 ), aquaporin 3 (AQP3 ), keratin 1 (KRT1), and keratin 10 (KRT10) and HA production. Real-time polymerase chain reaction (real-time PCR) revealed that hDSPC-CM increased HAS2 and AQP3 expression in NHEKs, and HA-ELISA revealed that hDSPC-CM increased HA production in NHEKs. On the other hand, hDSPC-CM decreased KRT1 and KRT10 expression in NHEKs. Conclusion: The results revealed that hDSPC-CM is a potent moisturizing cosmetic ingredient for epidermal moisturization. Based on this study, I anticipate further studies on mechanisms underlying the effects of hDSPC-CM on epidermal keratinocytes to develop not only cosmetics but also healthcare medicines.
No takes yet. Share an insight, caveat, or question.
Joong Hyun Shim (2018) studied this question.
Synapse has enriched 5 related papers. Consider them for comparative context: