Comparison of Lactate Transport in Astroglial Cells and Monocarboxylate Transporter 1 (MCT 1) Expressing Xenopus laevis Oocytes

Stefan Bröer; Basim Rahman; Gioranni Pellegri; Luc Pellerin; Jean‐Luc Martin; Stephan Verleysdonk; Bernd Hamprecht; Pierre J. Magistretti

doi:10.1074/jbc.272.48.30096

November 1, 1997Journal of Biological ChemistryOpen Access

Comparison of Lactate Transport in Astroglial Cells and Monocarboxylate Transporter 1 (MCT 1) Expressing Xenopus laevis Oocytes

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Key Points

To identify the molecular carrier responsible for lactate transport in astroglial cells and verify its kinetic properties by comparison with heterologously expressed monocarboxylate transporter 1 (MCT1).
Measured saturable [14C]lactate uptake kinetics and inhibitor sensitivity in primary rat astroglial cell cultures across varying substrate concentrations and pH conditions.
Assessed MCT1 and MCT2 mRNA expression in primary cultures of astroglia and neurons using reverse transcriptase-polymerase chain reaction and Northern blotting.
Cloned rat MCT1 cDNA, expressed it in Xenopus laevis oocytes, and compared transport kinetics directly to astroglial lactate uptake.
Astroglial lactate transport was mediated by a single saturable system with a Km of 7.7 mM and a Vmax of 250 nmol/(min × mg of protein), which was inhibited by monocarboxylates, α-cyano-4-hydroxycinnamate, and p-chloromercuribenzenesulfonate.
Astroglial cultures predominantly expressed MCT1 mRNA, whereas neuron-rich primary cultures selectively expressed MCT2 mRNA.
MCT1 expression in Xenopus laevis oocytes matched the affinity (Km 7.7 mM), pH dependence, and inhibitor profile of astroglial lactate transport, demonstrating MCT1 accounts for all glial transport characteristics.

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Implication

In vitro study reveals that MCT1 mediates astroglial lactate transport matching kinetics in Xenopus oocytes, supporting the astrocyte-neuron lactate shuttle hypothesis.

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Cite This Study

Bröer et al. (1997) studied this question.

synapsesocial.com/papers/6a1d17a85b7fddc35204d158 https://doi.org/10.1074/jbc.272.48.30096

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Comparison of Lactate Transport in Astroglial Cells and Monocarboxylate Transporter 1 (MCT 1) Expressing Xenopus laevis Oocytes

Key Points

No abstract yet

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Implication

Discussion

Cite This Study

Comparison of Lactate Transport in Astroglial Cells and Monocarboxylate Transporter 1 (MCT 1) Expressing Xenopus laevis Oocytes

Key Points

No abstract yet

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Implication

Discussion

Cite This Study

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