Imaging flow cytometry (iFCM) combines the spatial resolution of optical microscopes with the fast-sampling capability of flow cytometry.In contrast to recent advances in 2D-iFCMs for both analysis and cell sorting, the throughput and flow-speed in 3D-iFCM were limited.In this review, we overview the field, explain the challenges in speeding up 3D-iFCM, and report the recent developments of two 3D-iFCMs based on an idea of acquiring multiple optical sections of cells in a single frame of a pixel-arrayed camera.The two 3D-iFCM techniques demonstrate a record throughput in cells/sec and a record speed in meter/sec, respectively.
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Ota et al. (2022) studied this question.
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