What does this research mean for the field?

A newly developed loop-mediated isothermal amplification (LAMP) assay provides a rapid, visual method for detecting the fosfomycin resistance gene fosA with an overall sensitivity of 90.5% and specificity of 90.9%, though sensitivity is significantly lower in Escherichia coli compared to Klebsiella pneumoniae. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

The aim was to develop a rapid molecular method to detect the fosA resistance gene, associated with fosfomycin resistance.

June 1, 2026Scientific ReportsOpen Access

Development of loop-mediated isothermal amplification (LAMP)-based assay for rapid detection of fosfomycin resistance gene fosA

View Full Paper

Ask AI

Bookmark

Authors

NSNazareno ScacciaUniversidade de São Paulo INInneke Marie van der Heijden NatárioInstituto de Medicina Tropical LFLeticia Silva FigueiredoInstituto de Medicina Tropical

Discussion

Member takes

Overview

Randomized trial developed a LAMP assay to detect fosfomycin resistance in bacteria, implying better infection treatment options.

Key Points

The aim was to develop a rapid molecular method to detect the fosA resistance gene, associated with fosfomycin resistance.
Developed a LAMP-based assay to detect the fosA gene using 63 bacterial strains.
Evaluated primer concentration, reaction time, sensitivity, and specificity of the assay.
Detection methods included visual inspection and electrophoresis.
Overall sensitivity of the LAMP assay was 90.5% and specificity was 90.9%.
For Klebsiella pneumoniae, sensitivity was 100%; specificity not determined due to lack of negative samples.
In Escherichia coli, sensitivity was 43% and specificity was 90.9%.

Cite This Study

Scaccia et al. (2026) studied this question.

synapsesocial.com/papers/6a1d21e502fbce9130637d6b https://doi.org/10.1038/s41598-026-52613-5

View Full Paper

Ask AI

Bookmark

June 1, 2026Scientific ReportsOpen Access

Development of loop-mediated isothermal amplification (LAMP)-based assay for rapid detection of fosfomycin resistance gene fosA

View Full Paper

Ask AI

Bookmark

Authors

NSNazareno ScacciaUniversidade de São Paulo INInneke Marie van der Heijden NatárioInstituto de Medicina Tropical LFLeticia Silva FigueiredoInstituto de Medicina Tropical

Discussion

Member takes

Overview

Randomized trial developed a LAMP assay to detect fosfomycin resistance in bacteria, implying better infection treatment options.

Key Points

The aim was to develop a rapid molecular method to detect the fosA resistance gene, associated with fosfomycin resistance.
Developed a LAMP-based assay to detect the fosA gene using 63 bacterial strains.
Evaluated primer concentration, reaction time, sensitivity, and specificity of the assay.
Detection methods included visual inspection and electrophoresis.
Overall sensitivity of the LAMP assay was 90.5% and specificity was 90.9%.
For Klebsiella pneumoniae, sensitivity was 100%; specificity not determined due to lack of negative samples.
In Escherichia coli, sensitivity was 43% and specificity was 90.9%.

Cite This Study

Scaccia et al. (2026) studied this question.

synapsesocial.com/papers/6a1d21e502fbce9130637d6b https://doi.org/10.1038/s41598-026-52613-5

View Full Paper

Ask AI

Bookmark