What does this research mean for the field?

The bimolecular rate constant for the reaction of adenosine deaminase with adenosine is at the encounter-controlled limit, whereas its reaction with poor substrates is limited by other processes, and the binding of transition-state analog inhibitors is also viscosity-dependent. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

June 2, 1987Biochemistry

Adenosine deaminase: viscosity studies and the mechanism of binding of substrate and of ground- and transition-state analog inhibitors

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Population

Adenosine deaminase enzyme system (in vitro)

Design

Preclinical

Authors

LKLinda C. KurzWashington University in St. Louis EWEmma WeitkampUniversity of the West of England CFCarl FriedenWashington University in St. Louis

Discussion

Member takes

Implication

Advances kinetic models of adenosine deaminase; leaves open translation to cardiovascular adenosine signaling.

Structured PICO

Population

Adenosine deaminase enzyme system (in vitro)

Intervention

Viscosogenic agents (sucrose and ficoll)

Outcome

Hydrolysis rates of adenosine and 6-methoxypurine riboside, and rates of association and dissociation of ground-state and transition-state analogue inhibitorssurrogate

The reaction of adenosine deaminase with adenosine is encounter-controlled, as demonstrated by its dependence on microscopic viscosity.

Cite This Study

Kurz et al. (1987) studied this question.

synapsesocial.com/papers/6a1d292b750575be8d2f3cc7 https://doi.org/10.1021/bi00385a012

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June 2, 1987Biochemistry

Adenosine deaminase: viscosity studies and the mechanism of binding of substrate and of ground- and transition-state analog inhibitors

View Full Paper

Ask AI

Bookmark

Population

Adenosine deaminase enzyme system (in vitro)

Design

Preclinical

Authors

LKLinda C. KurzWashington University in St. Louis EWEmma WeitkampUniversity of the West of England CFCarl FriedenWashington University in St. Louis

Discussion

Member takes

Implication

Advances kinetic models of adenosine deaminase; leaves open translation to cardiovascular adenosine signaling.

Structured PICO

Population

Adenosine deaminase enzyme system (in vitro)

Intervention

Viscosogenic agents (sucrose and ficoll)

Outcome

Hydrolysis rates of adenosine and 6-methoxypurine riboside, and rates of association and dissociation of ground-state and transition-state analogue inhibitorssurrogate

The reaction of adenosine deaminase with adenosine is encounter-controlled, as demonstrated by its dependence on microscopic viscosity.

Cite This Study

Kurz et al. (1987) studied this question.

synapsesocial.com/papers/6a1d292b750575be8d2f3cc7 https://doi.org/10.1021/bi00385a012

Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

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