Interleukin-6 contributes to myocardial damage in pregnant rats with reduced uterine perfusion pressure

Figure 1.. Rats were subjected to reduced uterine perfusion pressure (RUPP) or sham operation and then intraperitoneally injected with interleukin (IL)-6 (50 ng/day) for 5 days. A, Expression of IL-6 in cardiac tissue was assayed by immunohistochemistry. Scale bar: 100 μm. B, Expression of IL-6 in serum was measured by ELISA. Experiments were repeated at least three times (n=6). Data are reported as means±SD. *P<0.05 vs control, #P<0.05 vs RUPP (one-way ANOVA).

Figure 2.. Rats were subjected to reduced uterine perfusion pressure (RUPP) or sham operation and then intraperitoneally injected with interleukin (IL)-6 (50 ng/day) for 5 days. A-C, Concentrations of cardiac troponin I (cTnI), creatine kinase isoenzyme (CK-MB), and myoglobin (Mb) in serum were detected by ELISA. D and E, Expressions of brain natriuretic peptide (BNP) in cardiac tissue and serum were detected by immunohistochemistry and ELISA. Scale bar: 100 μm. Experiments were repeated at least three times (n=6). Data are reported as means±SD. *P<0.05 vs control, #P<0.05 vs RUPP (one-way ANOVA).

Figure 3.. Rats were subjected to reduced uterine perfusion pressure (RUPP) or sham operation and then intraperitoneally injected with interleukin (IL)-6 (50 ng/day) for 5 days. A, Arterial pressure was monitored with a pressure transducer connected to an arterial pressure-recording device. B-D, Myocardial systolic peak velocity (Sm), early diastolic velocity (Ea), and late diastolic velocity (Aa) in three cardiac cycles were measured using tissue Doppler echocardiography. E, Early diastolic/late diastolic velocities ratio (E/Ea). All experiments were repeated at least three times (n=6). Data are reported as means±SD. *P<0.05 vs control, #P<0.05 vs RUPP (one-way ANOVA).

Figure 4.. Rats were subjected to reduced uterine perfusion pressure (RUPP) or sham operation and then intraperitoneally injected with interleukin (IL)-6 (50 ng/day) and tocilizumab (8 mg/kg) for 5 days. A, Expression of IL-6 in cardiac tissue was assayed by immunohistochemistry. Scale bar: 100 μm. B-D, Expression of IL-6, brain natriuretic peptide (BNP), and creatine kinase isoenzyme (CK-MB) in serum was measured by ELISA. E, Early diastolic/late diastolic velocities ratio (E/Ea). All experiments were repeated at least three times (n=6). Data are reported as means±SD. *P<0.05 vs control, #P<0.05 vs RUPP (one-way ANOVA).