Some conditions for the synthesis of phospholipase C by Bacillus cereus have been investigated. Nutrient broth dialysate, containing only substances of low molecular weight, was a convenient growth medium when starting material for the purification of phospholipase C was desirable. An unidentified low molecular medium component completely inhibited the synthesis of phospholipase C. Sporulation and growth rate were not detectably influenced by the presence or absence of the enzyme.
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Otnæss et al. (1972) studied this question.
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