Binding of Short Oligonucleotides to RNA: Studies of the Binding of Common RNA Structural Motifs to Isoenergetic Microarrays

Binding of short oligonucleotides to RNA is important for many biological processes. On the basis of RNAi phenomena, antisense, and ribozyme approaches, it is useful in the inhibition of biological functions. To be considered as potential therapeutics, oligonucleotides must bind strongly and selectively to a complementary fragment of target RNA. Microarray technologies also involve the binding of oligonucleotide probes to DNA or RNA. Herein, the hybridization of common structural motifs of RNA, i.e., hairpins, internal loops, bulges, 3'- and 5'-dangling ends, and pseudoknots to isoenergetic microarray probes is presented. The analysis demonstrates that microarray probes bind to bulges, internal loops, and dangling ends as expected. Probes may also bind to terminal helixes, however, possibly due to the rearrangement of base pairs. These results suggest that isoenergetic microarray mapping can provide data to facilitate and improve RNA secondary structure prediction. However, optimal results require combination with chemical and/or enzymatic mapping.