Key result
In patients with established coronary artery disease, flow-mediated dilation was positively associated with endothelial eNOS Ser1177 phosphorylation (r = 0.290, P = 0.037) and senescence markers p16 and p21.
Why the study?
Data were limited examining associations between endothelial dysfunction pathways and direct in vivo bioassay measures of endothelial function in CAD patients.
Cross-Sectional (n=56)
No
Answers are grounded in this paper and cite their sources.
In patients with established CAD, endothelium-dependent vasodilation is associated with eNOS Ser 1177 phosphorylation and senescence markers, but not with markers of oxidative stress, suggesting a distinct regulatory mechanism compared to preclinical populations.
Purpose Endothelial dysfunction is an early and integral event in the development of atherosclerosis and coronary artery disease (CAD). Reduced NO bioavailability, oxidative stress, vasoconstriction, inflammation and senescence are all implicated in endothelial dysfunction. However, there are limited data examining associations between these pathways and direct in vivo bioassay measures of endothelial function in CAD patients. This study aimed to examine the relationships between in vivo measures of vascular function and the expression of atherogenic risk-modulating proteins in endothelial cells (ECs) isolated from the radial artery of CAD patients. Methods Fifty-six patients with established CAD underwent trans-radial catheterization. Prior to catheterization, radial artery vascular function was assessed using a) flow-mediated dilation (FMD), and b) exercise-induced dilation in response to handgrip (HE%). Freshly isolated ECs were obtained from the radial artery during catheterization and protein content of eNOS, NAD(P)H oxidase subunit NOX2, NFκB, ET-1 and the senescence markers p53, p21 and p16 were evaluated alongside nitrotyrosine abundance and eNOS Ser 1177 phosphorylation. Results FMD was positively associated with eNOS Ser 1177 phosphorylation ( r = 0.290, P = 0.037), and protein content of p21 ( r = 0.307, P = 0.027) and p16 ( r = 0.426, P = 0.002). No associations were found between FMD and markers of oxidative stress, vasoconstriction or inflammation. In contrast to FMD, HE% was not associated with any of the EC proteins. Conclusion These data revealed a difference in the regulation of endothelium-dependent vasodilation measured in vivo between patients with CAD compared to previously reported data in subjects without a clinical diagnosis, suggesting that eNOS Ser 1177 phosphorylation may be the key to maintain vasodilation in CAD patients.
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Tryfonos et al. (2022) conducted a cross-sectional in Coronary artery disease (n=56). Observational (no intervention) was evaluated on Correlation between flow-mediated dilation (FMD) and eNOS Ser1177 phosphorylation (r = 0.290, p=0.037). In patients with established coronary artery disease, flow-mediated dilation was positively associated with endothelial eNOS Ser1177 phosphorylation (r = 0.290, P = 0.037) and senescence markers p16 and p21.
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