Genomic upregulation of cardiac Cav1.2α and NCX1 by estrogen in women

Figures

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  Fig. 1. Comparison of protein expression between the base (B) and apex (A) of the left ventricular epicardium in humans. Protein samples from the base (B) and the apex (A) of the LV epicardium were obtained from 3 men, 3 women, and 3 postmenopausal women and were probed with antibodies to compare the relative expression of Cav1.2α, NCX1, SERCA2a, RyR2, Nav1.5, and hERG. Panel a shows the protein densities which were normalized with respect to GAPDH. Panel b is a histogram of normalized band intensities expressed as base-apex percent differences derived from an analysis of myocardium from 6 men, 11 women, and 6 postmenopausal women

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  Fig. 2. a Western blots of base-apex differences in channel protein expression in male and female rabbit left ventricular epicardium. b Base-apex percent differences in protein expression on the rabbit left ventricular epicardium

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  Fig. 3. Comparison of protein expression between the epicardium (Ep) and endocardium (En) of the left ventricular myocardium in human hearts. Protein samples from the epicardium (Ep) and endocardium (En) of the LV were obtained from 3 men, 3 women, and 3 postmenopausal women and were probed with antibodies to compare the relative expression of Cav1.2α, NCX1, SERCA2a, RyR2, Nav1.5, and hERG. Panel a shows the protein densities which were normalized with respect to GAPDH. Panel b is a histogram of normalized band intensities expressed as epi-endo percent differences derived from an analysis of myocardium from 6 men, 11 women, and 6 postmenopausal women

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  Fig. 4. Effect of E2 incubation on ICa,L and INCX of female human iPS-CMs. a ICa,L current-voltage relationship from cardiac myocytes derived from hiPS cells was measured in cells cultured with 1 nM E2 (filled circles, n = 13) and compared to those cultured with vehicle (empty circles, n = 15). ICa,L was significantly increased by E2 incubation. (*P < 0.05). b INCX was measured as a function of voltage in female human iPS-CMs that were incubated with estradiol (filled circles, n = 5) and compared to iPS-CMs incubated with the vehicle (control) (empty circles, n = 5). There was a significant increase in INCX when incubated with E2. *P < 0.05

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  Fig. 5. Effect of E2 incubation on ICa,L and INCX of male human iPS-CMs. a Effect of 17-β-estradiol on ICa,L in male iPS-CMs. ICa,L was measured as described in the methods in male iPS-CMs following treatment with 17-β-estradiol (filled circles) and vehicle (empty circles). 17-β-estradiol treatment displayed a small but not significant increase in ICa,L as compared to vehicle treatment. b Effect of 17-β-estradiol on INCX in male iPS-CMs. Shown are the averaged current density for INCX obtained from male iPS-CMs treated with 17-β-estradiol (filled circles) and vehicle (empty circles)