6-Hydroxymelatonin sulphate, a unique urinary metabolite of melatonin, has been measured in human subjects for the first time using a chromatography mass spectrometry and an isotope labelled internal standard. Urinary levels in both males and females were similar and ranged from 25-125 nmol 24 h-1. A significant nocturnal elevations of 6-hydroxymelatonin sulphate was also established in a male subject.
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Fellenberg et al. (1980) studied this question.
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