Answers are grounded in this paper and cite their sources.
Dengue virus plaque formation on BHK-21 cell microplate cultures was described. The clear plaques were visible usually 5 days after incubation in a CO-2 incubator at 37 degrees. The cells cultured in a 3-oz bottle were sufficient to prepare two microculture plates which were usually ready for use after 1-2 days of cultivation in the CO-2 incubator at 37 degrees. The overall procedures were easy and of economic advantage.
No takes yet. Share an insight, caveat, or question.
Fujita et al. (1975) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: