Dengue Virus Plaque Formation on Microplate Cultures and Its Application to Virus Neutralization

Dengue virus plaque formation on BHK-21 cell microplate cultures was described. The clear plaques were visible usually 5 days after incubation in a CO-2 incubator at 37 degrees. The cells cultured in a 3-oz bottle were sufficient to prepare two microculture plates which were usually ready for use after 1-2 days of cultivation in the CO-2 incubator at 37 degrees. The overall procedures were easy and of economic advantage.