Separation of Myosin Heavy Chain Isoforms in Rat Skeletal Muscles by Gradient Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

This study was conducted to investigate the electrophoretic condition for the separation of a novel myosin heavy chain isoform, designated as HCIId, in rat skeletal muscles by gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis. HCIId was separated on a polyacrylamide (5-8%)/glycerol (30-40/0) gradient gel when electrophoresis was performed at 150V for about 18 h at 8C. HCIId was observed in most fast-twitch muscles. Of those muscles, the deep portion of the masseter muscle contained the most abundant HCIId and one of the masseter