Antimicrobial peptide-based magnetic separation combined with multiplex PCR for the simultaneous detection of multiple foodborne pathogens in food samples

Key Points

• This research aims to improve the detection of foodborne pathogens in food samples using advanced molecular techniques.
• Developed a broad-spectrum bacterial enrichment strategy using antimicrobial peptide-modified magnetic beads.
• Combined the method with multiplex PCR to detect five foodborne pathogens simultaneously.
• Focused on fruit juice samples to eliminate food matrix interference.
• Achieved a 100-fold improvement in detection sensitivity compared to conventional PCR.
• Successfully detected five foodborne pathogens in fruit juice samples.
• Eliminated food matrix interference, enhancing test accuracy.