Different mechanisms of desensitization of adenylate cyclase by isoproterenol and prostaglandin E1 in human fibroblasts. Role of regulatory components in desensitization.

IThe guanine nucleotide binding component of adenylate cyclase has been referred to as N (2, 3), G/F (4), and G (5) by various investigators.* T h e abbreviations used are: PGEI, prostaglandin El; N, regulatory component of adenylate cyclase; C, catalytic component of cyclase; ISO, (-)-isoproterenol; MIX, methylisobutyl xanthine; SDS, sodium dodecyl sulfate; EDTA, ethylenediaminetetraacetate; IHYP, iodohydroxybenzylpindolol; Gpp(NH)p, guanyl-5'-yl-imidodiphosphate. 5312