Key result
A protocol was outlined for the characterization of progenitor cell subsets from peripheral blood using high aldehyde dehydrogenase activity and flow cytometry.
Why the study?
Examining circulating pro-vascular progenitor cell frequency and function is integral to understanding aberrant blood vessel homeostasis in individuals with cardiometabolic disease.
Provides a protocol for isolating and characterizing circulating pro-vascular progenitor cells from human blood to study cardiometabolic disease.
Answers are grounded in this paper and cite their sources.
May aid cardiometabolic progenitor research; leaves open clinical translation pending validation.
The examination of circulating pro-vascular progenitor cell frequency and function is integral in understanding aberrant blood vessel homeostasis in individuals with cardiometabolic disease. Here, we outline the characterization of progenitor cell subsets from peripheral blood using high aldehyde dehydrogenase (ALDH) activity, an intracellular detoxification enzyme previously associated with pro-vascular progenitor cell status. Using this protocol, cells can be examined by flow cytometry for ALDH activity and lineage restricted cell surface markers simultaneously. For complete details on the use and execution of this protocol, please refer to Terenzi et al. (2019) and Hess et al. (2019, 2020).
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Terenzi et al. (2021) studied Cardiometabolic disease. Protocol for characterizing progenitor cell subsets using high ALDH activity was evaluated. A protocol was outlined for the characterization of progenitor cell subsets from peripheral blood using high aldehyde dehydrogenase activity and flow cytometry.
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