Membraneous fractions were isolated from human stratum corneum. The fractions obtained after frequent reextraction with urea and 2 ME at pH 9.0 (A), and further digestion of (A) by trypsin (B) were compared using electron microscopy and amino acid analysis. Low molecular weight proteins or amino peptides were solubilized from fraction (A) following digestion with trypsin, chymotrypsin and pronase P. The amounts of solubilized material showed a plateau after 30 minutes of digestion with trypsin. A small fraction of contaminants thought to be the fibrous and interfibrous materials were still observed in Fraction (A) by electron‐microscopy and SDS gel electrophoresis, but no remarkable contaminants were observed in Fraction (B), which was composed of trilaminar membraneous parts including the marginal band. Fraction (A) contained 43 half cystine residues per 1000 amino acid residues, whereas Fraction (B) contained 81 half cystine residues per 1000 amino acid residues.
No takes yet. Share an insight, caveat, or question.
Manabe et al. (1981) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: