Facile synthesis of Cu-CuFe2O4nanozymes for sensitive assay of H2O2and GSH

Artificial enzymes have drawn substantial research interest from the scientific community due to their advantages over natural enzymes. However, majority of artificial enzymes exhibit low affinity towards H2O2, which means that a high H2O2 concentration is needed for the oxidation of a substrate such as 3,3',5,5'-tetramethylbenzidine (TMB) to blue-colored oxTMB. With this concern, Cu-CuFe2O4 was facilely synthesized, wherein, Cu0 accelerates the redox capacity of Cu-CuFe2O4 as well as the electron transfer between CuFe2O4 and H2O2. These materials induce excellent activity as a peroxidase. Cu-CuFe2O4 shows high affinity towards H2O2 with lower Michaelis-Menten constant (Km) than the reported values for ferrites and Horseradish enzyme (HRP). Moreover, it took only 5 min to detect hydrogen peroxide (H2O2) and glutathione (GSH) through a colorimetric assay using Cu-CuFe2O4. Compared with CuFe2O4, the limit of detection (LOD) is about 90-fold lower for H2O2 using Cu-CuFe2O4. In addition, Cu-CuFe2O4 shows high stability as a nanozyme. Thus, the mechanism of the peroxidase-like nanozyme Cu-CuFe2O4 is proposed.