Gnaphalium polycephalum is a homeopathic medicinal plant widely used in the management of neuralgic conditions like sciatica. However, there is little scientific information about its phytochemical composition and chromatographic profile. The present study was conducted to investigate the phytochemical constituents and HPLC fingerprint profile of Gnaphalium polycephalum. Phytochemical screening was first performed on the mother tincture by standard qualitative methods. The chromatographic fingerprint was established by high-performance liquid chromatography (HPLC) analysis. Phytochemical analysis revealed the presence of flavonoids, tannins, and volatile oils. The HPLC chromatogram showed several peaks at different retention times, indicating the presence of different chemical constituents, providing a characteristic fingerprint profile. The data obtained from this study are useful for the identification, quality control, and standardization of Gnaphalium polycephalum, which can be used as a homeopathic remedy.
Shiny et al. (Fri,) studied this question.