Exploring the GM-CSF Histidine Triad as a Modulator of Structure, Molecular Motion, and Ligand Binding

Key Points

• The aim is to investigate how the GM-CSF histidine triad affects its structure and binding properties.
• Molecular dynamics simulations were used to analyze GM-CSF behavior.
• Experimental assays tested ligand interactions with GM-CSF variants containing histidine triad modifications.
• The presence of the histidine triad significantly altered GM-CSF's ligand binding affinity.
• GM-CSF variants exhibited distinct molecular motion patterns, influencing immune response.