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June 3, 2026Advanced Synthesis & Catalysis0 citations

A Highly Efficient Enzymatic Method for the Sustainable and Stereoselective Production of 5α‐H Key Intermediates for Steroidal Pharmaceuticals

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CLChi LuYWYue WangRZRui Zhang

Key Points

  • This research aims to develop a highly efficient enzymatic method for producing 5α‐H intermediates used in steroidal pharmaceuticals.
  • Identified 5α-reductase (5αRed‐9) from a Prevotellaceae bacterium.
  • Catalyzed the reduction of androstenedione with >99% de and broad substrate scope.
  • Performed preparative-scale reaction to isolate 5α‐AD.
  • Isolated 5α‐AD in 90% yield and 99% purity.
  • Achieved 13.3 gL −1 d −1 space‐time yield.
  • Demonstrated selectivity advantages over traditional chemical methods.

Abstract

A 5α‐reductase (5αRed‐9) from a Prevotellaceae bacterium was identified to catalyze the quantitative reduction of androstenedione with >99% de and exhibit a broad substrate scope. In the preparative‐scale reaction, 5α‐AD was isolated in 90% yield and 99% purity, with 13.3 gL −1 d −1 space‐time yield. This biocatalytic process overcomes the selectivity limitations of chemical methods, offering an efficient and sustainable strategy for industrial‐scale production.

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Cite This Study

Lu et al. (2026) studied this question.

synapsesocial.com/papers/6a1fc6f7dee9eb8c0dce7cfehttps://doi.org/10.1002/adsc.70572
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