Comparative Proteomics of Lipid Transport Proteins and Assessment of Oxidative Stress Parameters in Acute Ischemic Stroke and Healthy Control

Background: Stroke is one of the major causes of mortality worldwide. Ischemic stroke occurs due to blood vessel blockage that limits blood supply to the brain. The present study aims to identify the serum proteins in terms of its lipid transportation function and the level of oxidative stress parameters. The study was done in human blood samples of ischemic stroke patients and healthy control participants. The sample size used was 26 in each group for oxidative stress study and 3 per group for mass spectrometric protein analysis. The study was designed as a comparative analysis of oxidative stress parameters between control and ischemic stroke. The in gel tryptic digestion followed by Liquid chromatography – mass spectrometry (LC-MS-MS) analysis was done to identify the differentially expressed serum proteins between control and stroke. Students t test was used for comparison between groups and p value less than 0.05 (p<.05) considered as significant. The study observed that the antioxidant parameters such as catalase and glutathione had lowered activity in ischemic stroke group compared to healthy control and malondialdehyde levels were higher in ischemic stroke compared to control. From the in gel tryptic digestion, we observed that proteins linked to lipid transport and inflammation had differential expression between stroke and control. The serum proteins which were differentially altered in 25 KDa region in electrophoretogram between control and stroke had functional association with lipid transportation process and stress associated inflammatory roles.