In silico analysis revealed that two AT2R mutants (rs868939201 and rs1042852794) significantly alter protein conformation and affect the binding affinity of C21 and EMA401 compared to the wild type.
Computational modeling identifies specific AT2R polymorphisms that alter the binding of targeted small molecules, highlighting the need to consider genetic variation in drug development.
For more than a century, the renin-angiotensin system (RAS) has been acknowledged for playing a crucial part in the physiological control of arterial pressure, as well as sodium and fluid balance. It is now generally acknowledged that one of the receptor of RAS system i.e. angiotensin type 2 receptor (AT2R) functions as a repair system during pathophysiologic circumstances and performs a significant protective role. Efforts have been made previously to design suitable agonist and antagonist molecules to potentially modulate AT2R. One of the agonists and antagonists, named C21 and EMA401, has been studied in a number of pathological conditions. Additionally, a wide panel of single nucleotide polymorphisms (SNPs) has been reported for AT2R, which might potentially affect the efficacy of these molecules. Therefore, computational investigations have been carried out to analyze all the SNPs (1151) reported in NCBI to find potential SNPs affecting the active site of AT2R, as this domain is still unexplored. Structures of these polymorphic forms were modeled, and in silico drug interaction studies with C21 and EMA401 were carried out. The two mutants (rs868939201 and rs1042852794) that significantly affect the binding affinity as that of the wild type were subjected to molecular dynamics simulations. Our analysis of native and mutant AT2R and their complexes with C21 and EMA401 indicated that the occurrence of these mutations affects the conformation of the protein and has affected the binding of these ligand molecules. The study’s findings will aid in the development of better, more versatile medications in the near future, and also in vitro and in vivo studies might be planned in accordance with recent findings.Communicated by Ramaswamy H. Sarma
Sharma et al. (Fri,) reported a other. C21 and EMA401 vs. Wild type AT2R was evaluated on Binding affinity and protein conformation. In silico analysis revealed that two AT2R mutants (rs868939201 and rs1042852794) significantly alter protein conformation and affect the binding affinity of C21 and EMA401 compared to the wild type.