Impedance spectroscopy (EIS) has been proposed as the transduction principle for detecting the hybridization of DNA complementary strands. The resistance offered to the electrochemical reaction acts as the working signal, allowing an unlabelled gene assay. In our experiments, atypically large DNA oligonucleotides (110 base pairs) were used to determine the influence of ionic strength on the response of the DNA biosensor. In addition, the EIS response that was obtained by the hybridization process in the presence or absence of 6‐mercapto‐1‐hexanol (MCH) was also evaluated. Finally, we extended these studies to probes and duplexes of various lengths observing a linear relationship between duplex length and the response (expressed as the R CT value).
No takes yet. Share an insight, caveat, or question.
Revenga‐Parra et al. (2010) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: