High‐Resolution N⁶‐Methyladenosine (m⁶A) Map Using Photo‐Crosslinking‐Assisted m⁶A Sequencing

N 6 ‐methyladenosine (m 6 A) is an abundant internal modification in eukaryotic mRNA and plays regulatory roles in mRNA metabolism. However, methods to precisely locate the m 6 A modification remain limited. We present here a photo‐crosslinking‐assisted m 6 A sequencing strategy (PA‐m 6 A‐seq) to more accurately define sites with m 6 A modification. Using this strategy, we obtained a high‐resolution map of m 6 A in a human transcriptome. The map resembles the general distribution pattern observed previously, and reveals new m 6 A sites at base resolution. Our results provide insight into the relationship between the methylation regions and the binding sites of RNA‐binding proteins.