The detection of p30 by means of an indirect thin-layer immunoassay (TIA) is described. Extracts from 20 samples can be analyzed in approximately 2 h with a detection limit of approximately 50 ng. The p30 protein was detected in seminal stains which had been stored at room temperature for six months and at 130°C for 4 h. Blood, saliva, urine, perspiration, and tears did not interefere with the method. The reliability of the method was demonstrated in a blind study.
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Masibay et al. (1984) studied this question.
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