the reader can find descriptions of a recent StonyBrook/ Berkeley/Cornell two-dimensional imaging of a yeast cell by this technique. The present work is aimed at extending the technique to the three-dimensional imaging of a cell. However, the usual method of doing that, namely rotating the specimen into many orientations in the x-ray beam, has not as yet given sufficiently good threedimensional diffraction data to allow the work to go forward, the largest problem being the difficulty of preventing unwanted change in the specimen through the extended exposure to a hostile environment of x-rays and in some cases high vacuum and/or extreme cold. The present paper discusses possible methods of dealing with this problem, including the lowering of exposure to just two specimen orientations as needed for stereoscopic 3D presentation. Thanks in this work go to many, starting exactly 60 years ago, at IUCr1, or IUCr 1948, under Professor Ewald as the first IUCr president.
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D. Sayre (2008) studied this question.