Angiotensin II stimulates DNA and protein synthesis in vascular smooth muscle cells from human arteries

Cultured vascular smooth muscle cells derived from resistance arteries (< 300 microm diameter) from subcutaneous gluteal biopsies of healthy subjects (n = 6) and human aortic vascular smooth muscle cells (studied between passages 3 and 6).

Angiotensin II (10(-12) - 10(-6) mol/l), evaluated in the absence and presence of losartan (10(-5) mol/l), PD123319 (10(-5) mol/l), or PD98059 (10(-5) mol/l).

Control (untreated cells) and endothelin-1.

Vascular smooth muscle cell hyperplasia (DNA synthesis measured by 3H-thymidine incorporation) and cell hypertrophy (protein synthesis measured by 3H-leucine incorporation).surrogate