Key result
Reaction of actin and rabbit subfragment 1 with a carbodiimide cross-linking reagent forms a major doublet that is a 1:1 complex.
The study confirms that the major doublet formed by cross-linking actin and subfragment 1 is a 1:1 complex.
Informs actomyosin structural models; leaves open in vivo relevance to human cardiac muscle.
The interaction of actin and rabbit subfragment 1 has been reexamined by using a carbodiimide cross-linking reagent. A major doublet with an apparent molecular weight of 134 000 as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis has been identified. A minor product of much higher molecular weight is also formed. Stoichiometry determinations using [3H]actin and [14C]subfragment 1 indicate that the major doublet is a 1:1 complex of actin and subfragment 1. This result confirms that reported by Sutoh (1983) [ Sutoh , K. (1983) Biochemistry 22, 1579-1585]
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Heaphy et al. (1984) studied this question. Carbodiimide cross-linking reagent was evaluated on Stoichiometry of covalent actin-subfragment 1 complexes. Reaction of actin and rabbit subfragment 1 with a carbodiimide cross-linking reagent forms a major doublet that is a 1:1 complex.
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