High performance liquid chromatography (HPLC) has found wide application for qualitative and quantitative analysis of plant pigments. HPLC methods developed for the analysis of carotenoids, chlorophylls, flavonoids and betalaines are summarized. Compared to other chromatographic techniques the methods are precise, sensitive and less time consuming. Sample preparation prior to chromatography is generally minimal and often a simple extraction step is sufficient. The high resolution capability of HPLC has made the separation of closely related pigments possible and has provided a means for major advances into the study of plant pigments.
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Schwartz et al. (1982) studied this question.
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