PulseExploreJournal ClubDebatesTrendingResearchersJournals
Instagram
HomeExploreJournal ClubTrending
Synapse
⌘+K
Synapse
August 1, 1997Journal of Cardiovascular Pharmacology

Time Delay of Cell Death by Na+/H+-Exchange Inhibition in Regionally Ischemic, Reperfused Porcine Hearts

View Full Paper
Ask AI
Bookmark
Share

Why the study?

Does cariporide reduce infarct size and improve myocardial function in regionally ischemic, reperfused porcine hearts?

Population

20 thoracotomized pigs subjected to regional ischemia and 24 hours of reperfusion

Comparison

Cariporide 1 mg/kg injected intravenously 10 min… vs Control group subjected to 45 min of ischemia…

Design

Preclinical

Follow-up

24 hours of reperfusion

Key result

Pretreatment with the Na+/H+-exchange inhibitor HOE642 significantly decreased histochemical infarct size from 51.2% to 13.2% (p < 0.005) in porcine hearts subjected to 45 minutes of ischemia.

Authors

HKHermann H. KleinRBRainer M. BohleSPSibylle Pich

Discussion

Loading...

Member takes

Overview

Supports NHE inhibition for infarct reduction in porcine ischemia-reperfusion; leaves open human translation.

Key Points

  • This study aims to examine the impact of Na+/H+-exchange inhibition on cell death timing in ischemic and reperfused porcine hearts.
  • Injected HOE642 (1 mg/kg) into 14 thoracotomized pigs before occluding the left anterior descending coronary artery for varying durations.
  • Assessed infarct size with histology and evaluated myocardial function through sonomicrometry after 24 hours of reperfusion.
  • Included control animals (six) with 45 minutes of ischemia for comparison.
  • HOE642 reduced infarct size from 51.2% to 13.2% (p < 0.005) and histologic infarct size from 44.5% to 17.1% (p < 0.005) in pigs with 45 minutes of ischemia.
  • Recovery of systolic shortening improved from 2% to 12% (p = 0.02) after 24 hours of reperfusion.
  • Treatment with HOE642 enhanced ischemia/reperfusion tolerance by about 20-25 minutes, while longer ischemia durations did not show differential effects.

Structured PICO

Does cariporide reduce infarct size and improve myocardial function in regionally ischemic, reperfused porcine hearts?

P
Population
20 thoracotomized pigs subjected to regional myocardial ischemia and 24 hours of reperfusion.
I
Intervention
Cariporide (HOE642) 1 mg/kg injected intravenously 10 min before occlusion
C
Comparator
Control group (no pretreatment) subjected to 45 min of ischemia and 24 hours of reperfusion
O
Outcome
Infarct size (ratio of infarcted to ischemic myocardium) and regional myocardial function (recovery of regional systolic shortening)surrogate

Main Result

Absolute Event Rate: 13.2% vs 51.2%

p-value: p=< 0.005

Pretreatment with the Na+/H+-exchange inhibitor cariporide significantly reduces infarct size and improves functional recovery in a porcine model of myocardial ischemia and reperfusion, effectively delaying cell death.

Cite This Study

Klein et al. (1997) studied Myocardial ischemia and reperfusion (n=20). HOE642 (cariporide) vs. Control (no pretreatment) was evaluated on Histochemical infarct size (ratio of infarcted to ischemic myocardium) (p=< 0.005). Pretreatment with the Na+/H+-exchange inhibitor HOE642 significantly decreased histochemical infarct size from 51.2% to 13.2% (p < 0.005) in porcine hearts subjected to 45 minutes of ischemia.

synapsesocial.com/papers/6a201056d40b4a263065c648https://doi.org/10.1097/00005344-199708000-00013
View Full Paper
Ask AI
Bookmark
Share