An inhibitory protein for Mg2+-activated actomyosin ATPase from rabbit skeletal muscle was prepared from frozen chicken gizzard and purified by DEAE-Sephadex chromatography and gel filtration. 2. The inhibition by this protein was released by the addition of skeletal muscle troponin C and was independent of gizzard tropomyosin. 3. Localization of the inhibitory protein in gizzard muscle tissue and gizzard thin filaments was demonstrated by immunohistological techniques and immunodiffusion tests.
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Natasha Spellman (2007) studied this question.