A sequential model which describes the kinetic behavior of multilayer immobilized-enzyme filter reactors for systems obeying the Michaelis-Menten scheme and various types of inhibition by product and by substrate is proposed. The model was varified experimentaly using a single enzyme-filter-substrate system-urease bound to nylon-filter disks acting on urea-in different buffers to obtain the various inhibition patterns.
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Goldstein et al. (1983) studied this question.
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