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August 9, 2023Circulation ResearchOpen Access

PROX1 prevents myxomatous valve degeneration in mice by antagonizing PDGF-B signaling via FOXC2.

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Why the study?

Effective pharmacological treatments for cardiac valve disease are lacking, and whether the lymphatic and venous valve transcription factors PROX1 and FOXC2 regulate cardiac valve development and disease was unknown.

Population

Prox1 ΔVEC mice, isolated valve cells, and human mitral valve prolapse and aortic valve insufficiency samples

Comparison

Conditional deletion of Prox1 vs controls

Design

Preclinical animal and in vitro mechanistic study with human tissue validation

Key result

PROX1 antagonizes PDGF-B signaling partially via FOXC2 to maintain extracellular matrix composition and prevent myxomatous degeneration of cardiac valves in mouse models.

Authors

YHYen‐Chun HoXGXin GengAOAnna O’Donnell

Discussion

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Member takes

Overview

Does not support clinical use of PROX1 modulation; leaves open therapeutic targeting of this pathway in human valve disease.

Structured PICO

P
Population
Prox1 ΔVEC mice, isolated valve endothelial and interstitial cells, and human samples of mitral valve prolapse and aortic valve insufficiency
I
Intervention
Conditional deletion of Prox1, conditional knockdown of FOXC2, conditional overexpression of PDGF-B, and pharmacological inhibition of PDGF-B signaling with imatinib
O
Outcome
Valve structure and function (thickness, myxomatous changes, stenosis) and molecular expression of FOXC2 and PDGF-Bsurrogate

PROX1 antagonizes PDGF-B signaling partially via FOXC2 to maintain extracellular matrix composition and prevent myxomatous degeneration of cardiac valves, suggesting a potential therapeutic target.

Cite This Study

Ho et al. (2023) studied Cardiac valve disease (myxomatous degeneration). Prox1 deletion in valvular endothelial cells vs. Wild-type mice was evaluated on Valve structure and functioning (myxomatous degeneration and stenosis). PROX1 antagonizes PDGF-B signaling partially via FOXC2 to maintain extracellular matrix composition and prevent myxomatous degeneration of cardiac valves in mouse models.

synapsesocial.com/papers/6a201f4d76ec520ee9a1b124https://doi.org/10.1161/circresaha.123.323027
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