A versatile and inexpensive microbatch crystallization apparatus with an integrated thermal gradient is introduced. The device is designed to fit onto automated microscope stages for timed image acquisition. The thermal gradient is established through an aluminum plate machined to house commercially available microplates with a standard 96-well footprint. The novelty of the design includes online monitoring of crystal growth without interfering with the established thermal gradient. The temperature stability and unidirectional temperature gradient were demonstrated. Hen egg white lysozyme (HEWL) was used as a model macromolecule to observe the effect of temperature variations on crystal habit and the number and size of crystals produced.
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Bartling et al. (2005) studied this question.
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