Key result
Monoclonal Antibody Capture ELISA (MACELISA) demonstrated strong correlation with virus neutralization test results for TGEV serodiagnosis, outperforming indirect ELISA in sensitivity and specificity.
Why the study?
Does MACELISA improve the sensitivity and specificity of TGEV serodiagnosis compared to indirect ELISA and VNT?
Does MACELISA improve the sensitivity and specificity of TGEV serodiagnosis compared to indirect ELISA and VNT?
MACELISA is a highly sensitive and specific assay for TGEV serodiagnosis, making it an advisable first step in testing.
May support MACELISA for TGEV serodiagnosis in animals; leaves open routine adoption pending further validation.
An enzyme-linked immunosorbent assay (ELISA) in which the antigen is captured to the plate by monoclonal antibodies (MACELISA) was developed for the detection of antibodies to transmissible gastroenteritis virus (TGEV). The viral antigen was semipurified from TGEV-infected cells by simple ultracentrifugation. MACELISA results with 258 field sera were compared with those of a standard indirect ELISA and with the virus neutralization test (VNT). Sensitivity, specificity, and kappa values of MACELISA indicated a strong correlation with VNT results, whereas an indirect ELISA was less sensitive and much less specific than VNT. The serologic response of 4 pigs orally inoculated and intraperitoneally boostered with TGEV was compared using the 3 tests. Its sensitivity, specificity, and ability to use unpurified antigen make the MACELISA the advisable first step in TGEV serodiagnosis.
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Lanza et al. (1993) studied Transmissible gastroenteritis virus (TGEV) (n=258). Monoclonal Antibody Capture ELISA (MACELISA) vs. Indirect ELISA and Virus Neutralization Test (VNT) was evaluated on Sensitivity, specificity, and kappa values compared to VNT. Monoclonal Antibody Capture ELISA (MACELISA) demonstrated strong correlation with virus neutralization test results for TGEV serodiagnosis, outperforming indirect ELISA in sensitivity and specificity.