In vitro administration of α-B crystallin corrected high cardiomyocyte stiffness in failing human myocardium by shifting the passive tension-sarcomere length relation downward to baseline levels.
p-value: p=<0.05
Background— Cardiomyocytes with a less distensible titin and interstitial collagen contribute to the high diastolic stiffness of failing myocardium. Their relative contributions and mechanisms underlying loss of titin distensibility were assessed in failing human hearts. Methods and Results— Left ventricular tissue was procured in patients with aortic stenosis (AS, n=9) and dilated cardiomyopathy (DCM, n=6). Explanted donor hearts (n=8) served as controls. Stretches were performed in myocardial strips, and an extraction protocol differentiated between passive tension (F passive ) attributable to cardiomyocytes or to collagen. F passive -cardiomyocytes was higher in AS and DCM at shorter muscle lengths, whereas F passive -collagen was higher in AS at longer muscle lengths and in DCM at shorter and longer muscle lengths. Cardiomyocytes were stretched to investigate titin distensibility. Cardiomyocytes were incubated with alkaline phosphatase, subsequently reassessed after a period of prestretch and finally treated with the heat shock protein α-B crystallin. Alkaline phosphatase shifted the F passive –sarcomere length relation upward only in donor. Prestretch shifted the F passive –sarcomere length relation further upward in donor and upward in AS and DCM. α-B crystallin shifted the F passive –sarcomere length relation downward to baseline in donor and to lower than baseline in AS and DCM. In failing myocardium, confocal laser microscopy revealed α-B crystallin in subsarcolemmal aggresomes. Conclusions— High cardiomyocyte stiffness contributed to stiffness of failing human myocardium because of reduced titin distensibility. The latter resulted from an absent stiffness-lowering effect of baseline phosphorylation and from titin aggregation. High cardiomyocyte stiffness was corrected by α-B crystallin probably through relief of titin aggregation.
Franssen et al. (Tue,) conducted a other in Aortic stenosis and dilated cardiomyopathy (n=23). α-B crystallin vs. Baseline/untreated state was evaluated on Passive tension (Fpassive) to sarcomere length relation (p=<0.05). In vitro administration of α-B crystallin corrected high cardiomyocyte stiffness in failing human myocardium by shifting the passive tension-sarcomere length relation downward to baseline levels.