Simple Computer-Aided Approach for the Analyses of the Nuclear-Magnetic-Resonance Spectra of Histones. Fractions Fl, F2al, F2B, Cleaved Halves of F2B and F2B middotDNA

A simple method for the computer simulation of high-resolution nuclear magnetic resonance spectra is described and applied to the analyses of salt-induced changes in the spectra of histones. The method is more objective than the earlier approach of visual examination of spectral changes. It allows the information contained in the spectral envelope to be used and has led to some modifications of the earlier proposals of the segments of histones involved in salt-induced conformational changes, interhistone interactions and interactions with DNA. A feature of these newer proposals is their self-consistency, particularly of the results obtained for F2B, the cleaved amino and carboxyl halves of F2B and of F2B · DNA.