A human homologue (hDREF/KIAA0785) of Drosophila DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation, was identified by BLAST search. Amino acid sequences corresponding to three regions highly conserved between two Drosophila species also proved to be very similar in the hDREF/KIAA0785 polypeptide. A consensus binding sequence (5′-TGTCG(C/T)GA(C/T)A) for hDREF/KIAA0785, determined by the CASTing method, overlapped with that for the Drosophila DREF (5′-TGTCGATA). We found hDREF/KIAA0785 binding sequences in the promoter regions of human genes related to cell proliferation. Analyses using a specific antibody revealed that an hDREF/KIAA0785 binds to the promoter region of the histone H1 gene. Co-transfection experiments with an hDREF/KIAA0785-expressing plasmid and a histone H1 promoter-directed luciferase reporter plasmid in HeLa cells revealed possible activation of the histone H1 promoter. Immunohistochemical analysis demonstrated that hDREF/KIAA0785 is localized in the nuclei. Although the expression level of the factor was found to be low in serum-deprived human normal fibroblasts, the amount was increased by adding serum to cultures and reached a maximum during S phase. RNA interference experiments targeting hDREF/KIAA0785 resulted in inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells. These results suggest that expression of hDREF/KIAA0785 may have a role in regulation of human genes related to cell proliferation. A human homologue (hDREF/KIAA0785) of Drosophila DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation, was identified by BLAST search. Amino acid sequences corresponding to three regions highly conserved between two Drosophila species also proved to be very similar in the hDREF/KIAA0785 polypeptide. A consensus binding sequence (5′-TGTCG(C/T)GA(C/T)A) for hDREF/KIAA0785, determined by the CASTing method, overlapped with that for the Drosophila DREF (5′-TGTCGATA). We found hDREF/KIAA0785 binding sequences in the promoter regions of human genes related to cell proliferation. Analyses using a specific antibody revealed that an hDREF/KIAA0785 binds to the promoter region of the histone H1 gene. Co-transfection experiments with an hDREF/KIAA0785-expressing plasmid and a histone H1 promoter-directed luciferase reporter plasmid in HeLa cells revealed possible activation of the histone H1 promoter. Immunohistochemical analysis demonstrated that hDREF/KIAA0785 is localized in the nuclei. Although the expression level of the factor was found to be low in serum-deprived human normal fibroblasts, the amount was increased by adding serum to cultures and reached a maximum during S phase. RNA interference experiments targeting hDREF/KIAA0785 resulted in inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells. These results suggest that expression of hDREF/KIAA0785 may have a role in regulation of human genes related to cell proliferation. Promoters of Drosophila genes related to DNA replication, such as those for the 180-kDa catalytic subunit of DNA polymerase α and proliferating cell nuclear antigen (PCNA), 1The abbreviations used are: PCNA, proliferating cell nuclear antigen; BEAF, boundary element-associated factor; Dm, Drosophila melanogaster; CR, conserved region; HEL, human embryonic lung fibroblast; RT, reverse transcription; HA, hemagglutinin; GST, glutathione S-transferase; PBS, phosphate-buffered saline; Pipes, 1,4-piperazinediethanesulfonic acid; DTT, dithiothreitol; EMSA, electrophoretic mobility shift assay. contain a common 8-bp palindromic sequence (5′-TATCGATA), named the DRE (DNA replication-related element) (1Hirose F. Yamaguchi M. Handa H. Inomata Y. Matsukage A. J. Biol. Chem. 1993; 268: 2092-2099Abstract Full Text PDF PubMed Google Scholar), in addition to E2F recognition sites (2Ohtani K. Nevins J.R. Mol. Cell. Biol. 1994; 14: 1603-1612Crossref PubMed Scopus (111) Google Scholar, 3Duronio R.J. O'Farrell P.H. Xie J.E. Brook A. Dyson N. Genes Dev. 1995; 9: 1445-1455Crossref PubMed Scopus (216) Google Scholar, 4Yamaguchi M. Hayashi Y. Matsukage A. J. Biol. Chem. 1995; 270: 25159-25165Abstract Full Text Full Text PDF PubMed Scopus (70) Google Scholar, 5Royzman I. Whittaker A.J. Orr-Weaver T.L. Genes Dev. 1997; 11: 1999-2011Crossref PubMed Scopus (140) Google Scholar). Our previous studies (6Yamaguchi M. Hirose F. Matsukage A. Genes Cells. 1996; 1: 47-58Crossref PubMed Scopus (37) Google Scholar, 7Sawado T. Hirose F. Takahashi Y. Sasaki T. Shinomiya T. Sakaguchi K. Matsukage A. Yamaguchi M. J. Biol. Chem. 1998; 273: 26042-26051Abstract Full Text Full Text PDF PubMed Scopus (78) Google Scholar) performed in vitro and in vivo suggested that the DRE sequence and the E2F binding sites function synergistically in activation of PCNA, DNA polymerase α, and dE2F genes. We found a specific DREF (DRE-binding factor) consisting of an 80-kDa polypeptide homodimer, and molecular cloning of its cDNA has allowed confirmation that DREF is a trans-activator for DRE-containing genes (8Hirose F. Yamaguchi M. Kuroda K. Omori A. Hachiya T. Ikeda M. Nishimoto Y. Matsukage A. J. Biol. Chem. 1996; 271: 3930-3937Abstract Full Text Full Text PDF PubMed Scopus (116) Google Scholar). An important role of the DRE/DREF regulatory system has been indicated by the finding that DRE/DREF is a target of some differentiation signals. The zen (zerknullt) gene encoding a homeodomain-containing protein, Zen, which is expressed in the dorsal region of the early embryo at the cellular blastoderm stage, is involved in differentiation of the amnioserosa and the optic lobe (9Doyle H.J. Kraut R. Levine M. Genes Dev. 1989; 3: 1518-1533Crossref PubMed Scopus (81) Google Scholar). Zen expression in cultured cells results in repression of DRE-containing genes by reducing the DREF activity (10Hirose F. Yamaguchi M. Matsukage A. J. Biol. Chem. 1994; 269: 2937-2942Abstract Full Text PDF PubMed Google Scholar). Thus, the DRE/DREF system may occupy a in and differentiation K. Mol. Cell. Biol. 1997; PubMed Scopus Google Scholar, PubMed Scopus Google Scholar) a function of DREF as an of the boundary element-associated factor which is involved in the boundary activity of the region of the Drosophila gene I. Whittaker A.J. Orr-Weaver T.L. Genes Dev. 1997; 11: 1999-2011Crossref PubMed Scopus (140) Google Scholar, K. Mol. Cell. Biol. 1997; PubMed Scopus Google Scholar, PubMed Scopus Google Scholar). of with and revealed of for the two to using a method, demonstrated that DREF to the sequences as that of binding between DREF and is important for the regulation of activity at the have in which expression of DREF was to the Y. Hirose F. Matsukage A. Yamaguchi M. PubMed Scopus Google Scholar). DREF a We found that was by a reduction of of the R. M. A. 1998; PubMed Google Scholar), T. R. Mol. Cell. Biol. PubMed Google Scholar), and T. N. J.E. J. PubMed Scopus Google Scholar), involved in reduction of a factor involved in Dev. PubMed Scopus Google Scholar), the F. N. M. Y. Matsukage M. Yamaguchi M. Mol. Cell. Biol. PubMed Scopus Google Scholar). These results suggest a that DREF activity is by that a role in of the such as those I. Whittaker A.J. Orr-Weaver T.L. Genes Dev. 1997; 11: 1999-2011Crossref PubMed Scopus (140) Google Scholar, K. Mol. Cell. Biol. 1997; PubMed Scopus Google Scholar, PubMed Scopus Google Scholar) and Dev. PubMed Scopus Google Scholar). its of a DRE/DREF system has been for cDNA cloning of DREF, a gene for DREF Drosophila Y. Hirose F. Matsukage A. Yamaguchi M. PubMed Scopus Google Scholar) and determined highly conserved regions in of species and Drosophila (8Hirose F. Yamaguchi M. Kuroda K. Omori A. Hachiya T. Ikeda M. Nishimoto Y. Matsukage A. J. Biol. Chem. 1996; 271: 3930-3937Abstract Full Text Full Text PDF PubMed Scopus (116) Google Scholar). of acid sequences for the two species allowed to three highly conserved and Y. Hirose F. Matsukage A. Yamaguchi M. PubMed Scopus Google Scholar). A BLAST with the acid sequence of a required for DNA binding and (8Hirose F. Yamaguchi M. Kuroda K. Omori A. Hachiya T. Ikeda M. Nishimoto Y. Matsukage A. J. Biol. Chem. 1996; 271: 3930-3937Abstract Full Text Full Text PDF PubMed Scopus (116) Google Scholar), a human DREF to as T. K. M. R. N. A. H. N. DNA 1998; PubMed Scopus Google Scholar). of acid sequences of and the two Drosophila revealed of and the as a human homologue of DREF and demonstrated DNA binding activity and of expression during the cell found that hDREF/KIAA0785 binds to the promoter region of the histone H1 gene and its promoter cells in with human embryonic lung cells at in and with cells of serum for to addition of with the hDREF/KIAA0785 binding sequence in the promoter region of human histone H1 gene to as at to with to A.J. M. PubMed Scopus Google Scholar, T. 1997; PubMed Scopus Google Scholar) and its sequences and and the the hDREF/KIAA0785 binding sequence and and and and and and and and and and and and corresponding to the binding sequence for hDREF/KIAA0785 in with for the sequence in with and an and a region of and used for the CASTing A cDNA for hDREF/KIAA0785 was by using and sites for in and in by with The promoter region to of the human histone H1 gene was by using and sites for in and in by with A cDNA for the human histone H1 gene T. 1997; PubMed Scopus Google Scholar) was by using and was DNA T. K. M. R. N. A. H. N. DNA 1998; PubMed Scopus Google Scholar) and used as a to a cDNA for The cDNA was with a DNA and the of to A plasmid hDREF/KIAA0785 was by the cDNA of hDREF/KIAA0785 by the using as a with and and the of the expression plasmid cDNA encoding the region acid of hDREF/KIAA0785 was by and the of The reporter plasmid was as The promoter region to of the human histone H1 gene A.J. M. PubMed Scopus Google Scholar) was by using DNA HeLa cells as with and and the of promoter which the promoter of the luciferase gene. the promoter was by with and The DNA was with a DNA and in by J. T. A Scholar) and using a of and of in as (8Hirose F. Yamaguchi M. Kuroda K. Omori A. Hachiya T. Ikeda M. Nishimoto Y. Matsukage A. J. Biol. Chem. 1996; 271: 3930-3937Abstract Full Text Full Text PDF PubMed Scopus (116) Google Scholar). of cells by in of and and by at for at to a to the was expressed and in the was used to antibody in a with by with in cell cell by and to in a and at for with and for at and with the antibody for at with with an at a of for at with was in a and with by and the with using a to the was with a and the in phase with the in the of the of as HeLa cells cultured in three with phosphate-buffered and for with of R. J. Biol. 1994; PubMed Scopus Google Scholar) and low at the with of of was performed as was as The with and by for at the of hDREF/KIAA0785 by in at of by for The and by low the in of the and the was by at for using a The to the of and by for in at and with for at The with normal at a in with normal serum for three with PBS, the with antibody at a in with normal serum for a three with PBS, the and by of hDREF/KIAA0785 with CASTing was by of of and of and and of the of DNA polymerase at for performed by adding to DTT, and of of of serum and and at for with at for an The by and the with with of the by and and by in of a of and of and and of polymerase by of at at and at by a with and in of a and The amount of was and a corresponding to was used in CASTing of the used as in an electrophoretic mobility shift binding to in of a and at with and with by as with and and and sites of the sequences of of HeLa nuclear was by a 11: PubMed Scopus Google Scholar). HeLa cells in by and with of A DTT, and to the cells for A was used to the cell and at at and in DTT, and the to nuclear by at for at for The was in DTT, by and at in The of the was performed as (1Hirose F. Yamaguchi M. Handa H. Inomata Y. Matsukage A. J. Biol. Chem. 1993; 268: 2092-2099Abstract Full Text PDF PubMed Google Scholar), with A DTT, and of DNA was used with DTT, and of DNA was for with the HeLa cell nuclear in of DNA as at of the HeLa cell nuclear and the was for experiments with the HeLa cell nuclear was with the antibody for in at The and DNA and cells at cells in for of luciferase reporter plasmid and of plasmid luciferase the of the gene promoter as an cells using PubMed Scopus (216) Google Scholar). the with the expression reporter with with as an The amount of expression plasmid was to by addition of DNA cells cultured for and luciferase activity was with a reporter system luciferase activity was to luciferase performed with at three plasmid RNA was using and of RNA was to using a RNA The using as The was at for for and for using and as the performed the of The in a with and using hDREF/KIAA0785 by The sequences for and to regions to and to with to the was used as a HeLa cells using J. A. K. T. PubMed Scopus Google Scholar), and cells for at cells in the of for The in for at and in at for was using an antibody and an The of for was to be for of Amino between hDREF/KIAA0785 and Drosophila genes for and (8Hirose F. Yamaguchi M. Kuroda K. Omori A. Hachiya T. Ikeda M. Nishimoto Y. Matsukage A. J. Biol. Chem. 1996; 271: 3930-3937Abstract Full Text Full Text PDF PubMed Scopus (116) Google Scholar, Y. Hirose F. Matsukage A. Yamaguchi M. PubMed Scopus Google Scholar). of the acid sequences allowed to three highly conserved and We have demonstrated that is a required for DNA binding and by analysis of the polypeptide (8Hirose F. Yamaguchi M. Kuroda K. Omori A. Hachiya T. Ikeda M. Nishimoto Y. Matsukage A. J. Biol. Chem. 1996; 271: 3930-3937Abstract Full Text Full Text PDF PubMed Scopus (116) Google Scholar). have also that be important for function using region the of the system F. Yamaguchi M. Matsukage A. Mol. Cell. Biol. PubMed Scopus Google Scholar). Thus, performed a BLAST using the acid sequence for as a a found by a of the and using the with human T. K. M. R. N. A. H. N. DNA 1998; PubMed Scopus Google Scholar). The polypeptide and to in the regions corresponding to and and and and highly conserved that be a human homologue of and is to as with also found by the BLAST using the acid sequence as a in polypeptide sequences T. K. M. R. N. A. H. N. DNA 1998; PubMed Scopus Google Scholar) and the DNA binding region of the Drosophila K. Mol. Cell. Biol. 1997; PubMed Scopus Google Scholar). of acid sequences of the revealed of and by and of acid DNA binding activity in the region be that T. F. A. M. A. Mol. PubMed Scopus Google Scholar) a cDNA and the gene encoding and the cDNA the DNA and the gene to be localized the and and the acid sequence of the with Drosophila DREF, hDREF/KIAA0785 in the of HeLa and (8Hirose F. Yamaguchi M. Kuroda K. Omori A. Hachiya T. Ikeda M. Nishimoto Y. Matsukage A. J. Biol. Chem. 1996; 271: 3930-3937Abstract Full Text Full Text PDF PubMed Scopus (116) Google Scholar, PubMed Scopus Google Scholar) have demonstrated that is a nuclear We a antibody hDREF/KIAA0785 by of serum with and analysis revealed the antibody to with an 80-kDa polypeptide in a HeLa cell of the antibody with resulted in the of protein, HeLa cells. for hDREF/KIAA0785 in hDREF/KIAA0785 is with a specific nuclear HeLa cells with of found in and the to with that at a of hDREF/KIAA0785 may be to nuclear of hDREF/KIAA0785 was also with cell of HeLa cells and nuclear by with and low in hDREF/KIAA0785 was in and nuclear was localized in the nuclear of hDREF/KIAA0785 was with of and HeLa cells with and which also nuclear to nuclear and the with of hDREF/KIAA0785 of and DNA the M. T. Hayashi Y. M. J. Biol. Chem. 1997; Full Text Full Text PDF PubMed Scopus Google Scholar). at hDREF/KIAA0785, and of hDREF/KIAA0785 in the of and and These results that hDREF/KIAA0785 is with in a and of for hDREF/KIAA0785 hDREF/KIAA0785 is localized in (8Hirose F. Yamaguchi M. Kuroda K. Omori A. Hachiya T. Ikeda M. Nishimoto Y. Matsukage A. J. Biol. Chem. 1996; 271: 3930-3937Abstract Full Text Full Text PDF PubMed Scopus (116) Google Scholar). the of hDREF/KIAA0785 to DNA and to which performed a of CASTing experiments with a with for which and a region of was with and with DNA was by and DNA was by of and DNA and The of the determined sequences of in The consensus sequence for hDREF/KIAA0785 is a palindromic the of sequence of of the binding sequence (8Hirose F. Yamaguchi M. Kuroda K. Omori A. Hachiya T. Ikeda M. Nishimoto Y. Matsukage A. J. Biol. Chem. 1996; 271: 3930-3937Abstract Full Text Full Text PDF PubMed Scopus (116) Google Scholar) and the recognition of K. Mol. Cell. Biol. 1997; PubMed Scopus Google Scholar) to hDREF/KIAA0785 binding activity in HeLa cell nuclear by the palindromic sequence by was and used for as a in two with HeLa cell nuclear and hDREF/KIAA0785 by experiments with of HeLa cell nuclear with the antibody the in a addition of normal The that two between and HeLa cell nuclear contain hDREF/KIAA0785 protein, is and the of two of the for the sequence by CASTing using the sequence for binding using HeLa cell nuclear A of with the hDREF/KIAA0785 binding and used for as in the two by adding an amount of of and and with a at the to of the hDREF/KIAA0785 binding sequence and The and with two in the region of the hDREF/KIAA0785 binding for the binding at and of and and and with the binding the in the of the binding and and These results that the sequence in the of the hDREF/KIAA0785 binding an important role in hDREF/KIAA0785 for Genes hDREF/KIAA0785 genes that be the of hDREF/KIAA0785 protein, the and using BLAST and the of and The BLAST revealed that sequences sequences with of the consensus in the human and results for promoter regions in I. found hDREF/KIAA0785 binding sequences in a of genes related to cell proliferation, as for A. Hirose F. Hayashi Y. K. Yamaguchi M. 1995; PubMed Scopus Google Scholar). We genes by its function as DNA replication (DNA DNA cell regulation of and be that also found DRE in the promoter regions of Drosophila genes to those for human J. Biol. Chem. Full Text PDF PubMed Google Scholar), DNA polymerase N. F. 1997; PubMed Scopus Google Scholar), R. Mol. Cell. Biol. 11: PubMed Scopus Google Scholar), R. Mol. Cell. Biol. PubMed Scopus Google Scholar), J. Biol. Chem. 1995; 270: Full Text Full Text PDF PubMed Scopus Google Scholar), and J. M. Google Scholar). The suggest that hDREF/KIAA0785 be a homologue of hDREF/KIAA0785 target a polymerase a polymerase a a polymerase polymerase nuclear a in a The of the H1 a with hDREF/KIAA0785 demonstrated that the expression of Drosophila genes related to DNA replication and cell (1Hirose F. Yamaguchi M. Handa H. Inomata Y. Matsukage A. J. Biol. Chem. 1993; 268: 2092-2099Abstract Full Text PDF PubMed Google Scholar, 7Sawado T. Hirose F. Takahashi Y. Sasaki T. Shinomiya T. Sakaguchi K. Matsukage A. Yamaguchi M. J. Biol. Chem. 1998; 273: 26042-26051Abstract Full Text Full Text PDF PubMed Scopus (78) Google Scholar, K. Hirose F. Sakaguchi K. Y. Matsukage A. 1996; PubMed Scopus Google Scholar, Y. Yamaguchi M. Hirose F. J. Matsukage A. J. Biol. Chem. 1996; 271: Full Text Full Text PDF PubMed Scopus Google Scholar, Y. Hayashi Y. Matsukage A. Yamaguchi M. 1997; Scopus Google Scholar), hDREF/KIAA0785 human DNA replication-related genes. We the histone H1 gene to as a sequence the hDREF/KIAA0785 binding sequence was found in its promoter which has been and for which transcriptional regulatory have been A.J. M. PubMed Scopus Google Scholar, T. 1997; PubMed Scopus Google Scholar). The hDREF/KIAA0785 binding sequence is at to with to the We the hDREF/KIAA0785 the binding sequence in the histone H1 promoter with the hDREF/KIAA0785 binding and used for in with the HeLa cell nuclear and the H1 as a for the and the by adding amount of H1 and the consensus hDREF/KIAA0785 binding sequence and by adding and the of the two by with the antibody in a addition of normal hDREF/KIAA0785 H1 the function of hDREF/KIAA0785 with to of the histone H1 its promoter A DNA region the histone H1 promoter to was by using DNA HeLa cells and a plasmid the luciferase reporter gene experiments with reporter plasmid and an plasmid revealed that expression of the the H1 promoter activity in a expression of a for hDREF/KIAA0785 encoding acid corresponding to the region in activation The results that hDREF/KIAA0785 the histone H1 gene promoter. of hDREF/KIAA0785 in of the of the histone H1 gene is during phase A.J. J. J. Biol. Chem. Full Text PDF PubMed Google Scholar, N. PubMed Scopus Google Scholar), of hDREF/KIAA0785 during the cell by We used cultures of normal human lung for be to a by serum the cell addition of We cell serum by cells with and to and by cells with which is as phase M. T. Hayashi Y. M. J. Biol. Chem. 1996; 271: Full Text Full Text PDF PubMed Scopus Google Scholar). revealed that of cells by of serum the cell adding serum to the in the amount of hDREF/KIAA0785 in cells of serum was in with that of cultures of cells to in cells at serum a maximum in and of cells also that expression of hDREF/KIAA0785 is in with the proliferating in the amount of nuclear with the antibody was in cells in serum-deprived serum the of in of of cells with hDREF/KIAA0785 and expression was similar to those of The of of hDREF/KIAA0785 expression serum to with the phase in the cell A and The results that expression of hDREF/KIAA0785 is during the The expression of the histone H1 gene serum was also by M. F. A. J. A. PubMed Scopus Google Scholar), the for histone H1 mRNA was in cells. of histone H1 mRNA was at serum and the expression level reached a maximum at of histone H1 mRNA was similar to that of hDREF/KIAA0785 of hDREF/KIAA0785 of DNA and the H1 The results suggest a that hDREF/KIAA0785 a role in performed RNA interference targeting hDREF/KIAA0785 in HeLa cells. hDREF/KIAA0785 and and the expression of hDREF/KIAA0785 analysis revealed that of hDREF/KIAA0785 to and by of and to also demonstrated that and for hDREF/KIAA0785 in expression of histone H1 mRNA by of and of histone H1 mRNA by and with that of the histone H1 gene be the of the of hDREF/KIAA0785 reduction using the cells to with and of cells a and that a cells with and A and and and and to Thus, that hDREF/KIAA0785 an important role in the identified as a human DREF homologue of acid sequences corresponding to and of of and and of acid in DNA binding suggest that may similar DNA sequence as determined the hDREF/KIAA0785 binding sequence in vitro by the CASTing using hDREF/KIAA0785 The sequence that to have the to hDREF/KIAA0785 is and a of sequence of the 8-bp consensus sequence for We hDREF/KIAA0785 DNA binding activity using HeLa cell nuclear and determined the required sequences by with a of as The results that the binding sequence with the to hDREF/KIAA0785 is and the sequence in the be important for hDREF/KIAA0785 is that the consensus binding sequence for hDREF/KIAA0785 a sequence in its in the promoter regions of genes in the PubMed Scopus Google Scholar). is found in of promoter regions PubMed Scopus Google Scholar). such as E2F binding and gene expression Genes Dev. 1989; 3: PubMed Scopus Google Scholar, Y. R. Biol. Chem. 1998; PubMed Scopus Google Scholar). such as to and Genes Dev. PubMed Scopus Google Scholar, Mol. Cell. Biol. 1998; PubMed Scopus Google Scholar). We results that hDREF/KIAA0785 to and its with the Hirose and N. in that is that hDREF/KIAA0785 a of genes and Our for the hDREF/KIAA0785 binding sequence in the human revealed its in the promoter regions of of genes involved in DNA replication, DNA cell and We have to be the for A. Hirose F. Hayashi Y. K. Yamaguchi M. 1995; PubMed Scopus Google Scholar), and some of the genes have been demonstrated to be the of the DRE/DREF system (1Hirose F. Yamaguchi M. Handa H. Inomata Y. Matsukage A. J. Biol. Chem. 1993; 268: 2092-2099Abstract Full Text PDF PubMed Google Scholar, 7Sawado T. Hirose F. Takahashi Y. Sasaki T. Shinomiya T. Sakaguchi K. Matsukage A. Yamaguchi M. J. Biol. Chem. 1998; 273: 26042-26051Abstract Full Text Full Text PDF PubMed Scopus (78) Google Scholar, K. Hirose F. Sakaguchi K. Y. Matsukage A. 1996; PubMed Scopus Google Scholar, Y. Yamaguchi M. Hirose F. J. Matsukage A. J. Biol. Chem. 1996; 271: Full Text Full Text PDF PubMed Scopus Google Scholar, Y. Hayashi Y. Matsukage A. Yamaguchi M. 1997; Scopus Google Scholar). The gene by that may be a homologue of and may in the transcriptional regulation of genes related to cell proliferation. We the human histone H1 gene a sequence the sequence the to the hDREF/KIAA0785 and is as a gene expression is with DNA have the region the of the histone H1 promoter and found two that be important in the S H1 transcription; an and a J. PubMed Scopus Google Scholar, Mol. Cell. Biol. PubMed Scopus Google Scholar, F. N. Mol. Cell. Biol. 1989; 9: PubMed Scopus Google Scholar, F. J. N. Genes Dev. 1989; 3: PubMed Scopus Google Scholar), at to and to A.J. M. PubMed Scopus Google Scholar). The hDREF/KIAA0785 binding is at to in a region that has been by the promoter region to of the histone H1 gene and is by We that hDREF/KIAA0785 binds to the human histone H1 gene promoter and its activity for the two experiments with antibody demonstrated that hDREF/KIAA0785 binds to the sequence at to revealed that expression of hDREF/KIAA0785 the histone H1 gene promoter activity in expression of a of hDREF/KIAA0785 Promoters of DNA human and H1 histone genes contain a E2F binding the expression of genes is in a of and have been identified in vitro using analysis of promoter regions of histone genes and the of and a A.J. M. A. A. 1996; PubMed Scopus Google Scholar, A.J. F. A. A. J. Cell. 1997; PubMed Scopus Google Scholar, F. A.J. Mol. Biol. 1998; PubMed Scopus Google Scholar), F. A.J. Mol. Biol. 1998; PubMed Scopus Google Scholar, F. A.J. H. T. 1995; PubMed Scopus Google Scholar, F. H. T. A.J. J. Biol. Chem. 1998; 273: Full Text Full Text PDF PubMed Scopus (81) Google Scholar), and F. N. Mol. Cell. Biol. 1989; 9: PubMed Scopus Google Scholar, F. J. N. Genes Dev. 1989; 3: PubMed Scopus Google Scholar) suggested to be involved in of human histone genes during to during the cell and of in regulation of cell histone gene expression have been for and A.J. F. A. A. J. Cell. 1997; PubMed Scopus Google Scholar, F. A.J. Mol. Biol. 1998; PubMed Scopus Google Scholar, F. H. T. A.J. J. Biol. Chem. 1998; 273: Full Text Full Text PDF PubMed Scopus (81) Google Scholar). and analysis that expression of hDREF/KIAA0785 is by adding serum to cultures of normal human fibroblasts, a maximum in S phase. previous finding that of in the DNA in the cells by DNA replication genes F. N. M. Y. Matsukage M. Yamaguchi M. Mol. Cell. Biol. PubMed Scopus Google Scholar), that hDREF/KIAA0785 may have an important role in of histone gene hDREF/KIAA0785 function in the histone H1 gene reduction of hDREF/KIAA0785 the histone H1 gene of hDREF/KIAA0785 resulted in reduction of histone H1 that hDREF/KIAA0785 the histone H1 gene found that the histone in the the histone H1 gene also have hDREF/KIAA0785 binding of hDREF/KIAA0785 resulted in inhibition of We have Y. Hirose F. Matsukage A. Yamaguchi M. PubMed Scopus Google Scholar) that expression of a of in cells of in the by of hDREF/KIAA0785 and also a of that hDREF/KIAA0785 be the hDREF/KIAA0785 as a human homologue of and demonstrated that be a transcriptional regulatory We also that the human histone H1 gene is of the of Although the in the that hDREF/KIAA0785 be the homologue of a that hDREF/KIAA0785 is the the that hDREF/KIAA0785 similar activity as We to that hDREF/KIAA0785 its DNA binding with that of studies genes with hDREF/KIAA0785 binding sequence of the function of We T. for cDNA and N. for the expression plasmid We also to M. for of the
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