Screening and Characterization of Achromobacter xylosoxidans isolated from rhizosphere of Jatropha curcas L. (Energy Crop) for plant-growth-promoting traits

Plant growth promoting rhizobacteria (PGPR) colonizes almost all the ecological niches in and around the plant roots and enhances plant growth and show profound impact upon plants productivity. In the present study we have isolated large number of bacterial isolates from the rhizosphere of non-edible oil seed plant Jatropha curcas (Common name: Physic nut; Family: Euphorbiaceae). Out of large number of isolates we have selected only four bacterial isolates (AKDJ1, AKDJ2, AKDJ3, and AKDJ4) on the basis of their multifarious PGP traits (bioflim production, ammonia production, indole acetic acid (IAA), phosphate solubilization, catalase enzyme and cellulase enzyme production). Out of four, the isolate AKDJ2 was characterized by various biochemical utilization tests (Citrate, lysine, ornithine, urease, phenylalanine, H2S production, nitrate reduction, glucose, lactose, adonitol, sorbitol, arabinose, and 35 different carbohydrate sources) and identified as Achromobacter xylosoxidans (Gene bank Accession no KX698100) which showed 99% similarity with Achromobacter xylosoxidans strain NBRC15126 by using 16S rDNA sequencing. We conclude that, bacterial isolates screened from the rhizosphere of plant could serve as a source of potential biofertilizer for improving the production of same and other crops under variety of stress conditions.