A system of versatile insertion plasmids was constructed that permits efficient delivery of the target sites of an ultra-rare-cutting endonuclease and the recombinase FLP into preselected sites of the bacterial genome. With the help of this system, the pathogenicity island LEE of the Escherichia coli O157:H7 genome was excised and isolated in vitro, deleted in vivo, rescued as a plasmid, and transferred into another strain.
No takes yet. Share an insight, caveat, or question.
Pósfai et al. (1997) studied this question.
Synapse has enriched one closely related paper. Consider it for comparative context: