July 1, 1975Proceedings of the National Academy of Sciences164 citationsOpen Access

Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus.

MEM J Ensinger SMScott A. Martin EPEnzo Paoletti

Key Points

Key points are not available for this paper at this time.

Abstract

RNA guanylyl and methyl transferases have been solubilized from vaccinia virus cores. The guanylyl transferase specifically adds a GMP residue to the 5'-terminus of unmethylated vaccinia virus mRNA to form the structures G(5')ppp(5')Gp- and G(5')ppp(5')Ap-. Studies with alpha-32PGTP and beta, gamma-32PGTP indicated that only the alpha-phosphate is transferred. In the presence of S-adenosylmethionine, the methyl transferases convert the blocked 5'-termini to m7G(5')ppp(5')Gmp- and m7G(5')ppp(5')Amp-. Similarly, the enzymes can modify synthetic poly(A) to form the structure m7G(5')ppp(5')Amp-.

AI से पूछें

Bookmark

View Full Paper

Cite This Study

Ensinger et al. (Tue,) studied this question.

synapsesocial.com/papers/6a2083687b2df09761f8988f https://doi.org/https://doi.org/10.1073/pnas.72.7.2525

AI से पूछें

Bookmark

View Full Paper