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November 1, 1994Journal of BacteriologyOpen Access

Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination

N. F. Tsinoremas Alan K. Kutach Carl A. Strayer Susan S. Golden

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Abstract

We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.

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Tsinoremas et al. (1994) studied this question.

synapsesocial.com/papers/6a20a65baa8e57945c6d9bb6 https://doi.org/10.1128/jb.176.21.6764-6768.1994

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